{"id":327,"date":"2021-06-30T10:18:31","date_gmt":"2021-06-30T14:18:31","guid":{"rendered":"http:\/\/sites.scranton.edu\/careerservices\/?p=327"},"modified":"2021-08-19T08:55:17","modified_gmt":"2021-08-19T12:55:17","slug":"microworld","status":"publish","type":"post","link":"https:\/\/sites.scranton.edu\/careerservices\/2021\/06\/30\/microworld\/","title":{"rendered":"MicroWorld"},"content":{"rendered":"<p>Through my research this summer, I hope to gain more confidence in my knowledge and abilities in the lab. Being in a research setting is very different from being in General Biology or Microbiology lab. You can be very confident in what you are learning in those labs, however, research requires a different way of thinking that\u2019s beyond just performing certain techniques for a grade. The switch in thinking is quite overwhelming, but I know it takes time and practice to nurture and, luckily, I\u2019m not the only one in the same position. Because there is five of us in our research team I\u2019m certain that we\u2019ll all be there for and help one another. Gaining more lab confidence will definitely help me in my career path because I\u2019m aiming to get my Ph.D. and that\u2019ll mean lots of time spent in a lab doing research.<\/p>\n<p>Our first experiment was to see if our bacteria, <em>Caulobacter crescentus<\/em>, could grow in the water that\u2019s available to us. We tested four different kinds of water: filtered (MQ), tap, deionized (DI), and office tank. The cultures incubated overnight to let the bacteria grow. Initially, we didn\u2019t get much growth. We only found bacteria in the media made with MQ and tap water through a simple stain, but it turned out that there was some sort of contamination in the MQ water because a species that is not <em>C. crescentus<\/em> grew. We did, however, get <em>C.<\/em> <em>crescentus<\/em> to grow in the tap water media. After one more night of incubation we did see growth in all the tubes.<\/p>\n<figure id=\"attachment_329\" aria-describedby=\"caption-attachment-329\" style=\"width: 298px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-329\" src=\"http:\/\/sites.scranton.edu\/careerservices\/files\/2021\/06\/ca1-298x300.jpg\" alt=\"\" width=\"298\" height=\"300\" srcset=\"https:\/\/sites.scranton.edu\/careerservices\/files\/2021\/06\/ca1-298x300.jpg 298w, https:\/\/sites.scranton.edu\/careerservices\/files\/2021\/06\/ca1-1018x1024.jpg 1018w, https:\/\/sites.scranton.edu\/careerservices\/files\/2021\/06\/ca1-150x150.jpg 150w, https:\/\/sites.scranton.edu\/careerservices\/files\/2021\/06\/ca1-768x772.jpg 768w, https:\/\/sites.scranton.edu\/careerservices\/files\/2021\/06\/ca1-624x628.jpg 624w, https:\/\/sites.scranton.edu\/careerservices\/files\/2021\/06\/ca1.jpg 1036w\" sizes=\"auto, (max-width: 298px) 100vw, 298px\" \/><figcaption id=\"caption-attachment-329\" class=\"wp-caption-text\">This is Caulobacter crescentus in the media made with tap water after one night of incubation. It can be identified because of C. crescentus\u2019s slightly curved shape, small size, and what it looks like when it divides.<\/figcaption><\/figure>\n<figure id=\"attachment_328\" aria-describedby=\"caption-attachment-328\" style=\"width: 300px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-328\" src=\"http:\/\/sites.scranton.edu\/careerservices\/files\/2021\/06\/ca2-300x300.jpg\" alt=\"This is Caulobacter crescentus in the media made with tap water after one night of incubation. It can be identified because of C. crescentus\u2019s slightly curved shape, small size, and what it looks like when it divides. \" width=\"300\" height=\"300\" srcset=\"https:\/\/sites.scranton.edu\/careerservices\/files\/2021\/06\/ca2-300x300.jpg 300w, https:\/\/sites.scranton.edu\/careerservices\/files\/2021\/06\/ca2-1024x1024.jpg 1024w, https:\/\/sites.scranton.edu\/careerservices\/files\/2021\/06\/ca2-150x150.jpg 150w, https:\/\/sites.scranton.edu\/careerservices\/files\/2021\/06\/ca2-768x768.jpg 768w, https:\/\/sites.scranton.edu\/careerservices\/files\/2021\/06\/ca2-624x624.jpg 624w, https:\/\/sites.scranton.edu\/careerservices\/files\/2021\/06\/ca2.jpg 1087w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><figcaption id=\"caption-attachment-328\" class=\"wp-caption-text\">This is the contaminated media made with MQ water after one night of incubation. From what I understand Dr. Randich belives this to be a Bacillus species because of its large rod shape and what it looks like when it divides.<\/figcaption><\/figure>\n<p>Christina Alfano &#8217;22<br \/>\nBiochemistry, Cell and Molecular Biology<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Through my research this summer, I hope to gain more confidence in my knowledge and abilities in the lab. Being in a research setting is very different from being in General Biology or Microbiology lab. You can be very confident in what you are learning in those labs, however, research requires a different way of [&hellip;]<\/p>\n","protected":false},"author":357,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[3,6,4,5],"class_list":["post-327","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-blog","tag-research","tag-royal-experience","tag-the-university-of-scranton"],"_links":{"self":[{"href":"https:\/\/sites.scranton.edu\/careerservices\/wp-json\/wp\/v2\/posts\/327","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.scranton.edu\/careerservices\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.scranton.edu\/careerservices\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.scranton.edu\/careerservices\/wp-json\/wp\/v2\/users\/357"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.scranton.edu\/careerservices\/wp-json\/wp\/v2\/comments?post=327"}],"version-history":[{"count":4,"href":"https:\/\/sites.scranton.edu\/careerservices\/wp-json\/wp\/v2\/posts\/327\/revisions"}],"predecessor-version":[{"id":359,"href":"https:\/\/sites.scranton.edu\/careerservices\/wp-json\/wp\/v2\/posts\/327\/revisions\/359"}],"wp:attachment":[{"href":"https:\/\/sites.scranton.edu\/careerservices\/wp-json\/wp\/v2\/media?parent=327"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.scranton.edu\/careerservices\/wp-json\/wp\/v2\/categories?post=327"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.scranton.edu\/careerservices\/wp-json\/wp\/v2\/tags?post=327"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}